Group Ⅰ but not Group Ⅱ NPV induces antiviral effects in mammalian cells |
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Authors: | LIANG Changyong SONG Jianhua HU Zhihong CHEN Xinwen |
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Institution: | 1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 2. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China |
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Abstract: | Nucleopolyhedrovirus (NPV) is divided into Group Ⅰ and Group Ⅱ based on the phylogenetic analysis. It has been reported that Group Ⅰ NPVs such as Autographa californica multiple NPV (AcMNPV) can transduce mammalian cells, while Group Ⅱ NPVs such as Helicoverpa armigera single NPV (HaSNPV) cannot. Here we report that AcMNPV was capable of stimulating antiviral activity in human hepatoma cells (SMMC-7721) manifested by inhibition of Vesicular Stomatitis virus (VSV) replication. In contrast, the HaSNPV and the Spodoptera exigua multiple NPV (SeMNPV) of group Ⅱ had no inhibitory effect on VSV. Recombinant AcMNPV was shown to induce interferons alpha/beta even in the absence of transgene expression in human SMMC-7721 cells, while it mediated transgene expression in BHK and L929 mammalian cells without an ensuing antiviral activity. |
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Keywords: | antiviral effect baculovirus VSV IFN-α/β mammalian cells transgene expression |
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