转座子挽救法对转座子突变菌株中插入位点的定位分析

为寻找谷氨酸棒杆菌转座子插入突变菌株中的转座子插入位点，采用了转座子挽救法对转座子及其插入位点附近的序列进行分离，并测定插入位点相邻DNA序列，获得了三个转座子插入位点DNA序列，其中一个是柠檬酸合成酶基因，另两个为目前未知基因，暂命名为orfA和orfB。该方法简便易行，是分析转座子插入位点的理想方法。

Analysis on Integration Sites of Transposon-insertion Mutant by Transposon Rescue

In order to define the chromosomal locus of the transposon integration in a mutant of Corynebacterium glutamicum, transposon rescue experiments were performed. By isolating parts of the transposon together with adjacent parts of the bacterial chromosome and subsequent sequencing of the adjacent parts,the mutant was found to have three transposon integrations, one just in front of the citrate synthase gene gltA, the other two in hitherto unknown open reading frames designated orfA and orfB. The transposon rescue experiments are proven to be a easy, practical and ideal way in analyzing the transposon integration sites.