Optimization of fermentation conditions for production of xylanase by a newly isolated strain,Penicillium thiersii ZH-19 |
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| Authors: | Cui Fengjie Li Yin Liu Zhiqiang Zhao Hui Ping Lifeng Ping Liying Yang Yinan Xue Yaping Yan Lijiao |
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| Affiliation: | (1) School of Food and Biotechnology, Jiangsu University, 2120013 Zhenjiang, People’s Republic of China;(2) Department of Plant Science, North Dakota State University, Fargo, ND 58105, USA;(3) Institute of Bioengineering, Zhejiang University of Technology, 310014 Hangzhou, People’s Republic of China;(4) Department of Chemical and Biological Science, NSF-I/UCRC Center for Biocatalysis and Bioprocessing of Macromolecules, Polytechnic Institute of New York University, Six Metrotech Center, Brooklyn, NY 11201, USA;(5) Department of Statistics, North Dakota State University, Fargo, ND 58105, USA;(6) Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, 310021 Hangzhou, People’s Republic of China;(7) College of Horticulture, Jilin Agricultural University, 130118 Changchun, People’s Republic of China;(8) College of Life Sciences, Zhejiang University, 310058 Hangzhou, People’s Republic of China; |
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| Abstract: | The objective of this study was to maximize production of xylanase by a newly isolated strain Penicillium thiersii ZH-19. Response surface methodology was employed to study the effects of significant factors such as pH, temperature, xylan concentration, and cultivation time, on the production of xylanase by Penicillium thiersii ZH-19. The optimal fermentation parameters for enhanced xylanase production were found to be pH 7.72, temperature 24.8°C, xylan 13.2 g l−1 and the fermentation time 125.8 h. The model predicted a xylanase activity of 75.24 U ml−1. Verification of the optimization showed that the maximum xylanase production reached 73.50 U mL−1 in the flask experiments and 80.23 U mL−1 in the scale of 15-L fermenter under the optimal condition. |
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| Keywords: | Identification Penicillium thiersii ZH-19 Response surface methodology Optimization Xylanase |
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