Localization of proteins forming the outer surface of isolated metaphase chromosomes. |
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| Authors: | N D Belyaev V G Budker V A Dubrovskaya A A Kim E V Kiseleva V N Sidorov |
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| Affiliation: | Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences. |
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| Abstract: | The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested. |
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