Heterobifunctional cross-linking of a monoclonal antibody with 2-methyl-N1-benzenesulfonyl-N4-bromoacetylquinonediimide |
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| Authors: | F A Liberatore R D Comeau R G Lawton |
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| Institution: | Medical Products Department, E.I. du Pont de Nemours & Company, Billerica, MA 01862. |
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| Abstract: | The Cyssor reagent, 2-methyl-N1-benzenesulfonyl-N4-bromoacetylquinonediimide, which will cleave a protein chain at Cys under acidic conditions, cross-linked unreduced and partially reduced antibody at pH 8.0. No cleavage of the antibody occurred suggesting that the Cyssor reagent may be useful with certain proteins as a heterobifunctional cross-linker. |
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