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Wound healing of human skin transplanted onto the nude mouse. II. An immunohistological and ultrastructural study of the epidermal basement membrane zone reconstruction and connective tissue reorganization
Authors:M Démarchez  D J Hartmann  D Herbage  G Ville  M Pruniéras
Affiliation:1. Cell Biology Department, Centre International de Recherches Dermatologiques, Sophia Antipolis, F-06565 Valbonne, France;2. Centre de Radioanalyse, Institut Pasteur, 13, Rue Domer, 69007 Lyon, France;3. Université Claude Bernard, Laboratoire d''histologie expérimentale, UA CNRS 244, 69622 Villeurbanne, France;4. Laboratoires de Recherche Fondamentale L''Oréal, 1, Avenue Saint-Germain, 93600 Aulnay-sous-Bois, France;1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, PR China;2. Tianshui Entry-Exit Inspection and Quarantine Bureau, Gansu, 741020, PR China;1. Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, University Hospital of Munich, Munich, Germany;2. Orthopaedie Oberland, Bavaria, Germany;1. Identification Center, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan;2. Fourth Biology Section, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan;3. Fifth Biology Section, National Research Institute of Police Science, 6–3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan;4. First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan;1. Institut Pasteur - CNRS URA 2582, Cell Polarity, Migration and Cancer Unit, 25 rue du Dr Roux, 75724 Paris Cedex 15, France;2. Université Pierre et Marie Curie, Cellule Pasteur UPMC, rue du Dr Roux, 75015 Paris, France
Abstract:The reconstruction of human epidermis during healing of human skin wounded after grafting onto the nude mouse was described in a previous paper (M. Démarchez, P. Sengel, and M. Pruniéras, 1986, Dev. Biol. 113, 90-96). The regeneration of the epidermal basement membrane zone (BMZ) and the reorganization of the connective tissue are the subjects of the present study. They were investigated by two complementary methods: electron microscopy to analyze the BMZ reorganization, and indirect immunofluorescence with species-specific and cross-reacting antibodies directed against laminin, bullous pemphigoid antigen, mouse or human collagens of types I or IV, human elastic fibers, fibronectin, fibrin, actin, and human vimentin, to examine the species origin and distribution of BMZ and connective tissue components during the regeneration process. It is reported that grafted human skin preserves its own immunological markers not only in the epidermis but also in the BMZ and dermis as well, and that, after injury, its regeneration proceeds according to the following sequence of overlapping events: production of a mouse granulation tissue; reepidermization by human cells; reconstruction of a BMZ with human characteristics; formation of a human neodermis. It is concluded that human skin grafted onto the nude mouse is able to regenerate its three structural compartments, namely, the epidermis, BMZ, and dermis. Interestingly, it appeared, also, that the connective tissue regeneration would be a two-step mechanism including the sequential formation of two tissues of distinct sources, namely, a granulation tissue and a neodermis.
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