CAPS-microsatellites: use of CAPS method to convert non-polymorphic microsatellites into useful markers |
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Authors: | Ayse Gul Ince Mehmet Karaca A Naci Onus |
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Institution: | (1) Faculty of Agriculture, Department of Horticulture, Akdeniz University, 07059 Antalya, Turkey;(2) Faculty of Agriculture, Department of Field Crops, Akdeniz University, 07059 Antalya, Turkey; |
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Abstract: | Microsatellites found in expressed sequence tags (ESTs) have several advantages in comparison to conventional microsatellites
developed from genomic DNA. Advantages of EST-microsatellites include better transferability between the species and lower
development cost of primer pairs. However, in many cases the amplified products of genomic DNA are larger than their expected
sizes based on ESTs and these markers have low level of polymorphism. In the present study we report on a new approach as
a modest modification of the cleavage amplified polymorphic sequence (CAPS) called CAPS-microsatellites. Using a total of
20 EST-microsatellite primer pairs on four Capsicum species and two Gossypium species we observed that all 20 monomorphic EST-microsatellites could be converted into polymorphic markers among the species
used. Further analyses using a total of 37 F2 individuals obtained from two Capsicum annuum L. lines indicated that CAPS-microsatellite markers usually segregated as co-dominant fashions, discriminating the homozygous
from heterozygous genotypes and could be used in genetic analyses of plant species. |
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