Regulation of sucrose storage by amino acid arginine in sugarcane cell suspension cultures |
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Authors: | Monica?Sachdeva Email author" target="_blank">A?P?S?MannEmail author S?K?Batta |
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Institution: | (1) Department of Biochemistry and Chemistry, Punjab Agricultural University, 141004 Ludhiana, Punjab, India |
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Abstract: | Sugarcane cell cultures were obtained from callus formed on explants derived from young expanding leaves of two early maturing
sugarcane varieties viz “CoJ83” and “CoJ86”. The cell cultures were varied with different arginine concentrations in the culture medium. For each
cultivar, sucrose content with 20 μM arginine in the culture medium decreased from 3 to 5 days and then increased to 10 days
after subculturing. Higher concentration of arginine in the culture medium (60 μM) decreased the sucrose content at different
days after subculturing and thus significantly stimulated sucrose mobilization. The activity of sucrose synthase and sucrose
phosphate synthase reached maximum while the activity of acid and neutral invertase was minimal in the culture medium with
20 μM arginine. Thus arginine at low concentration (20 μM) enables the cells to accumulate the higher level of sucrose. The
optimum level of amino acids can be utilized to regulate the in vivo activity of sucrose synthase, sucrose phosphate synthase and invertase to achieve maximum sucrose accumulation in sugarcane
storage tissue. |
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Keywords: | Sugarcane cell cultures sucrose synthase sucrose phosphate synthase invertase sucrose storage |
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