Evidence for a rapid structural change in TMV-A-protein near neutrality |
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Authors: | D Vogel |
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Institution: | Lehrstuhl für Biochemie II im Fachbereich Biologie der Universität, D-84 Regensburg, W. Germany |
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Abstract: | Titration of TMV-A-protein from pH 8 to 7 (20°C) or raising the temperature from 4° to 20°C (pH 7) produces, within a few minutes, a reversible change in the aromatic region of the CD-spectrum, before any extensive aggregation has taken place. This spectral change is solely a matter of the conditions of the solution and not of the history of the protein. There is no further CD-change during the slow aggregation process. Thus there must be some proton-uptake within the A-protein. The results are discussed with regard to the different interpretations of the role of A-protein or double-disc in the elongation-step of TMV-“in vitro” -self-assembly. |
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Keywords: | TMV Tobacco Mosaic Virus CD Circular Dichroism |
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