Rice callus growth, proline content and activity of proline and IAA oxidase under the influence of hydroxyproline and NaCl |
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Authors: | Vyjayanti A. Chauhan G. Prathapasenan |
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Affiliation: | (1) Department of Botany, M.S. University of Baroda, 390 002 Baroda, India |
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Abstract: | Calli of salt tolerant (Bhoora rata) and salt susceptible (GR11) rice varieties were cultured on Linsmaeir and Skoog’s medium containing LD50 concentration of NaCl (200 mM) and hydroxyproline (10 mM). Growth, proline content and activity of proline and IAA oxidases of the cultured tissues were determined at the end of 0, 2, 4, and 6 weeks of incubation. Hydroxyproline resistant calli of both rice varieties when cultured on Linsmaeir and Skoog’s medium containing hydroxyproline and NaCl showed increased dry weight and proline content as compared to NaCl stressed calli. The levels of proline and IAA oxidases were also low in the hydroxyproline resistant calli. |
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Keywords: | Hydroxyproline NaCl rice callus proline oxidase IAA oxidase |
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