Protein p0071, a major plaque protein of non-desmosomal adhering junctions,is a selective cell-type marker |
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Authors: | Ilse Hofmann Cäcilia Kuhn Werner W Franke |
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Institution: | (1) Joint Research Division Vascular Biology of the Medical Faculty Mannheim, University of Heidelberg, and the German Cancer Research Center (DKFZ) at Mannheim, CBTM, Ludolf-Krehl-Strasse 13–17, 68167 Mannheim, Germany;(2) Helmholtz Group for Cell Biology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, 69120 Heidelberg, Germany |
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Abstract: | Protein p0071, which originally was introduced as a member of the p120-subfamily of armadillo proteins, common to desmosomes and adhaerens junctions (AJs) and to several other cell structures (centrosomes, midbodies), has been localized by using a series of novel
mono- and polyclonal antibodies generated against various domains of the molecule. By protein analysis and immunolocalization
techniques, protein p0071 has been localized as a plaque protein in AJs of diverse epithelia and certain vascular endothelia,
in the composite junctions (areal compositae) of the intercalated disks of cardiomyocytes, and in the punctate or more extended AJs of the vast majority of cell culture
types examined, including mitotic states. Using these antibodies, we have also shown that this AJ protein occurs only rarely
or is even absent in tissues such as skeletal and smooth muscles, in a series of mesenchymal tissue cells, and in specific
desmosome-rich cells such as those of the upper layers of the epidermis and certain other stratified epithelia and Hassall
corpuscles of the thymus. We have also demonstrated that p0071 is absent from desmosomes. The occurrence of two major subtypes
of lymphatic endothelial cells, one with AJs containing p0071 and one without detectable p0071, is emphasized. Possible structural
and functional roles of p0071 are discussed in light of these new findings regarding its localization, and the addition of
p0071 to the armamentarium of cytodiagnostic cell-type markers is recommended.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
This study was supported by project grants from the German Ministry for Education and Research (BMBF) in a cooperative research
program entitled “Standardization of mesenchymal stem cells for regenerative medicine (START-MSC)” and from the Deutsche Krebshilfe
(project 10–2049) to W.W. Franke. |
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Keywords: | Protein p0071 p120-subfamily Cell-cell adhering junctions Puncta adhaerentia Desmosomes Human Borine Murine |
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