The microdetermination of 2,3-diphosphoglycerate |
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| Authors: | R Sasaki K Ikura E Sugimoto H Chiba |
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| Affiliation: | Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto, Japan |
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| Abstract: | A procedure for microestimation of 2,3-diphosphoglycerate, utilizing its role as coenzyme in the phosphoglycerate mutase reaction is described. The coenzymic activity was determined by assaying phosphoglycerate mutase polarimetrically without a coupled enzyme. This method is applicable to samples containing as little as 0.002 μmole of 2,3-diphosphoglycerate/ml. The content in various biological extracts was determined. |
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