Study of the effectiveness of methods of purification of metalloprotease produced by Bacillus subtilis

The effectiveness of the purification of proteolytic enzyme produced by B. subtilis production strain 3H in different nutrient media was evaluated. As revealed in this study, the use of the initial material obtained in peptone nutrient medium made it possible to obtain proteolytic enzyme with the highest specific activity by the methods of salting out, gel and ion-exchange chromatography, but the use of these methods on a production scale led to the deterioration of purity characteristics. Changes in the nutrient medium composition used for the cultivation of the production strain resulted in greater effectiveness of the purification methods. The highly purified preparation of metalloprotease with specific activity exceeding that of available commercial preparations more than twofold was obtained.