Electrochemical sterilization of bacteria adsorbed on granular activated carbon |
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Authors: | Tadashi Matsunaga Satoshi Nakasono Seiichi Masuda |
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Affiliation: | Unitat de Microbiología, Facultat de Farmàcia, Universitat de València, Spain. |
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Abstract: | Subcellular distribution of chitin synthetase has been studied in germ tubes of Candida albicans. Two fractions with synthetase activity were separated from cell homogenates: (i) a mixed membrane fraction where the enzyme, partly in an active form, is associated with the plasma membrane (isopycnic centrifugation of mixed membrane fraction on linear sucrose gradients resolved a unique peak of activity matching with [3H]ConA-labelled membranes at a buoyant density of 1.195 g/ml); and (ii) a cytoplasmic fraction containing fully zymogenic enzyme associated with particles whose buoyant density (determined by isopycnic centrifugation on linear sucrose gradients) depended on the cell breakage conditions. The actual cytoplasmic fraction-enzyme may correspond to particles with buoyant density 1.135 g/ml (chitosomes), whereas the enzyme particles with other densities (1.085 and 1.165 g/ml) probably originated during cell disruption, as has been reported previously to occur during the preparation of yeast cell homogenates. |
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Keywords: | Electrochemical sterilization Granular activated carbon Water treatment Growth inhibition |
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