Effects of density and cellular aging on collagen synthesis and growth kinetics in keloid and normal skin fibroblasts |
| |
Authors: | Barbara J McCoy John Galdun I Kelman Cohen |
| |
Institution: | (1) Division of Plastic Surgery, Medical College of Virginia, Virginia Commonwealth University, 23298 Richmond, Virginia |
| |
Abstract: | Summary Cell proliferation and collagen synthesis were analyzed in high, medium, and low density keloid and normal skin fibroblasts
and also at various times during the course of in vitro aging to expand previous findings of increased collagen synthesis
in keloid compared to normal skin fibroblasts. It was found that both keloid and normal fibroblasts (<20 population doublings)
responded similarly to high, medium, and low initial plating densities; however, sparsely plated keloid fibroblasts exhibited
a loss of replicative capacity earlier in their in vitro lifespan than did sparsely plated normal skin fibroblasts.
When analyzed at population doubling levels 2 to 38, collagen synthesis was elevated in keloid compared to normal skin fibroblasts
but decreased at the same rate in both cell types throughout this in vitro interval.
Supported by NIH Grant GM-20298. |
| |
Keywords: | keloid fibroblast collagen synthesis in vitro aging growth kinetics |
本文献已被 SpringerLink 等数据库收录! |
|