THE IN VIVO BIOSYNTHESIS OF CHOLESTEROL FROM (3RS,2R) [2-14C,2-3H]MEVALONIC ACID IN THE BRAINS OF RATS ON NORMAL AND PROTEIN DEFICIENT DIETS.1 THE STEREOCHEMISTRY OF TRITIUM ATOMS AT C-7 AND C-15

Abstract— 3RS.2R) [2-¹⁴C,2-³H]Mevalonic acid was injected into the midline thalamus section of the brain of 13-day old rats born and nursed by mothers fed isocaloric diets containing 8% and 25% casein. After 4.5 h. the rats were killed, the brains removed, and the biosynthesized cholesterols were isolated. It was determined that the overall outcome of biosynthetic events at C-7 and C-15 in cholesterol biosynthesized in the rat liver homogenates and in the midline thalamus of the brain of rats raised on the 8% and 25% casein diets is the same.