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Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain |
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| Authors: | Baek-Rock Oh Sun-Yeon Heo Sung-Mok Lee Won-Kyung Hong Jang Min Park You Ree Jung Dae-Hyuk Kim Jung-Hoon Sohn Jeong-Woo Seo Chul Ho Kim |
| Affiliation: | 1. Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk, 580-185, South Korea 2. Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Jeonbuk, 561-756, South Korea 3. Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Taejeon, 305-333, South Korea |
| Abstract: | Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, ?ldhA/pBR-iBO (ilvIH–ilvC–ilvD–kivd–adhA), produced 2-butanol (160 mg l?1) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320 mg l?1. This represents the first successful attempt to produce 2-butanol from crude glycerol. |
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