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NMR assignment of the nonstructural protein nsp3(1066–1181) from SARS-CoV |
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| Authors: | Pedro Serrano Margaret A Johnson Amarnath Chatterjee Bill Pedrini Kurt Wüthrich |
| Institution: | (1) Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., MB-44, La Jolla, CA 92037, USA;(2) Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., MB-44, La Jolla, CA 92037, USA |
| Abstract: | Sequence-specific NMR assignments of the globular core comprising the residues 1066–1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly 13C, 15N]-labeled protein. The backbone and side chain assignments are nearly complete, providing the basis for the ongoing NMR structure determination. A preliminary identification of regular secondary structures has been derived from the 13C chemical shifts. |
| Keywords: | Severe acute respiratory syndrome SARS coronavirus Nonstructural protein NMR structure determination |
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