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小麦叶片老化期间的内肽酶与H_2O_2的关系(英文)
引用本文:张志刚,芮琪,徐朗莱. 小麦叶片老化期间的内肽酶与H_2O_2的关系(英文)[J]. Acta Botanica Sinica, 2001, 0(2)
作者姓名:张志刚  芮琪  徐朗莱
作者单位:南京农业大学理学院!南京210095
基金项目:theNationalNaturalScienceFoundationofChina (396 70 438)
摘    要:研究了H2 O2 和蛋白水解酶在小麦 (TriticumaestivumL .cv.Yanmai15 8)叶片老化过程中的关系。小麦叶片老化期间 ,H2 O2 含量高的叶片中内肽酶活力也高。老化后期 ,内源H2 O2 迅速累积 ,内肽酶活力迅速上升 ;通过内肽酶同工酶电泳可检测到新增一种活力较强的内肽酶。用外源H2 O2 处理全展旗叶的内肽酶粗提液 ,随着H2 O2 浓度的升高 ,内肽酶活力先上升后下降。

关 键 词:内肽酶  H2O2  小麦叶片  老化

Relationship Between Endopeptidase and H_2O_2 During the Aging of Wheat Leaf
ZHANG Zhi_Gang,RUI Qi,XU Lang_Lai [WTBZ]. Relationship Between Endopeptidase and H_2O_2 During the Aging of Wheat Leaf[J]. , 2001, 0(2)
Authors:ZHANG Zhi_Gang  RUI Qi  XU Lang_Lai [WTBZ]
Affiliation:ZHANG Zhi_Gang,RUI Qi,XU Lang_Lai [WT6BZ]
Abstract:The relationship between hydrogen peroxide (H 2O 2) and endopeptidase(EP) in wheat ( Triticum aestivum L. cv. Yanmai 158) leaves was studied during natural and artificial aging. Rapid accumulation of endogenous H 2O 2 and marked increase of EP activity were observed during the later phase of aging. A new EP isozyme with higher activity was detected by electrophoresis on polyacrylamide gels containing denatured heamoglobin. With the increase of exogenous H 2O 2, the activity of EP increased at first and then decreased.
Keywords:endopeptidase  H 2O 2  wheat leaf  aging
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