| 小麦中两个肉桂酰辅酶A还原酶基因的分离和表达分析 |
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| 引用本文： | 蔺占兵,马庆虎,等.小麦中两个肉桂酰辅酶A还原酶基因的分离和表达分析[J].Acta Botanica Sinica,2001,43(10):1043-1046. |
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| 作者姓名： | 蔺占兵 马庆虎 |
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| 作者单位： | 中国科学院植物研究所，北京100093 |
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| 基金项目： | SupportedbythegrantsfromtheNationalSpecialProgramforTransgenicPlantResearchandCommercializationofChina (J99_A_0 31)，theNationalNaturalSci
enceFoundationofChina ( 30 0 70 0 67)andYoungScientistsGroupofLifeScienceandBiotechnologyInnovationoftheChineseAc |
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| 摘 要： | 肉桂酰辅酶A还原酶（CCR）负责催化木质素单体生物合成中最重要的代谢反应，它将类苯丙酸类代谢物转移到木质素的合成途径中，为了更好地了解木质素在小麦生长发育中的作用，从小麦（Triticum aestivum L.ev,H4564)中克隆了两个肉桂酰辅酶A还原酶的cDNA，相似性和进化关系的分析表明这两个cDNA片段分别属于不同的肉桂酰辅酶A还原酶，这两个cDNA片段分别命名为W－cr6和W-cr19,RT-PCR和Northen杂交结果证明，W－cr6基因主要在小麦的茎和叶中表达，W－cr19基因主要在根和茎中表达，上述结果表明在小麦的基因组中至少存在两类肉桂酰辅酶A还原酶基因。
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| 关 键 词： | 小麦 cDNA克隆 肉桂酰辅酶A还原酶 木质素 基因分离 |
Cloning and Expression Analysis of Two Wheat cDNAs Encoding Cinnamoyl-CoA Reductase |
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| Abstract: | Cinnamoyl_CoA reductase (CCR) is responsible for the first committed reaction in monolignol biosynthesis, which diverts phenylpropanoid_derived metabolites into the biosynthesis of lignin. To gain a better understanding of the lignin biosynthesis in wheat development, two cDNAs encoding CCR were identified from wheat ( Triticum aestivum L. cv. H4564). DNA sequence analyses indicated that the two cDNAs represent two classes of CCR. RT_PCR and Northern blot hybridization demonstrated that one of them, W_cr6, was expressed actively in stem and leaf tissue, the other one, W_cr19, was expressed in root and stem tissue. The results suggested that there are at least two genes encoded for CCR existing in wheat genome. |
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| Keywords: | Triticum aestivum cDNA cloning cinnamoyl_CoA reductase lignin |
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