番茄线粒体和内质网小分子热激蛋白基因的分子克隆

以热激处理的番茄（Lycopersicon esculentum Mill．）花为实验材料，构建了cDNA库，运用ＲＴ－ＰＣＲ方法克隆番茄粒体和内质网小分子热激蛋白cDNA，利用这两个保守区片段为探针，筛选cDNA库，获得线粒体和内质网小分子热激蛋白全序列cDNA。；通过分析线粒体和内质网小分子热激蛋白基因对温度的反应，发现小分子热激蛋白基因在番茄花中的热激应答温度低于它们在叶片中的热激应答温度，并且番茄叶片中的线粒体小分子热激蛋白基因还具有低温应答特性。对线粒体和内质网小分子热激蛋白基因的分子结构特点，小分子热激蛋白基因在番茄花中的特别热激应答温度的调控机理以及线粒体小分子热激蛋白的基因在中片中的低温度应答成因进行了讨论。

Molecular Cloning the Gene of Small Heat Shock Protein in the Mitochondria and Endoplasmic Reticulum of Tomato

A cDNA library was constructed with the heat shocked tomato (Lycopersicon esculentum Mill.) flowers and then was screened with the probes of mitochondrial and endoplasmic reticulum conservative regions that were cloned by using RT_PCR. The complete cDNAs of mitochondrial and endoplasmic reticulum small heat shock protein ( shsp ) were selected out from the cDNA library. Furthermore, the temperature responses of these shsp genes were determined. Northern hybridization showed that the heat response temperatures of both genes in tomato flower were lower than that in leaf and that mitochondria shsp in leaf was cold_inducible. In this paper, the molecular features of the cloned genes, the causes of the uncommon heat response temperatures of sHSP in flower and the cold inducible character of mitochondria shsp gene in leaf were discussed.