| 拟南芥耐低钾突变体的筛选及遗传分析 |
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| 引用本文： | 赵淑清,林春,等.拟南芥耐低钾突变体的筛选及遗传分析[J].Acta Botanica Sinica,2001,43(1):105-107. |
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| 作者姓名： | 赵淑清 林春 |
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| 作者单位： | [1]中国农业大学生物学院植物科学系，农业部植物生理化开放实验室，北京１０００９４ [2]中国农业大学生物学院植物 |
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| 基金项目： | SupportedbytheNationalNaturalScienceFoundationofChina ( 3 95 70 0 74，3 95 2 5 0 0 3，and 3 9873 95 6toWWH)andBeijingScienceFou |
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| 摘 要： | 利用乙酰甲基磺酸（ＥＭＳ）诱变方法，以幼苗根在重力作用下的弯曲生长为指标、筛选得到了拟南芥（Ａrabidopsis thaliana)耐低钾突变体。经过对突变体杂交后代的遗传分析证明，其中两株突变体的耐低钾性状为隐性单基因突变所致。鉴定、分离与植物耐低钾性状连锁的基因将有可能与对培育钾高效作物品种有重要意义。
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| 关 键 词： | 拟南芥 耐低钾 突变体 遗传分析 筛选 |
Isolation and Genetic Analysis of Arabidopsis Mutants with Low_K~ Tolerance |
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| Abstract: | Ethyl methane_sulfonate (EMS)_mutagenized Arabidopsis M 2 populations were screened in low_K medium using the root_bending assay. Forty_two putative low_K _tolerant ( lkt ) mutants were selected from 150?000 tested M 2 seedlings, and two of these mutants maintained their low_K _tolerant phenotype in their M 3 generations, respectively. Genetic analysis showed that either one of these two mutants has a monogenic recessive mutation in a nuclear gene, and that the two mutations in two independent mutants are allelic to each other. |
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| Keywords: | Arabidopsis thaliana low K _tolerance mutant |
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