A novel DNA polymerase inhibitor and a potent apoptosis inducer: 2-mono-O-acyl-3-O-(alpha-D-sulfoquinovosyl)-glyceride with stearic acid |
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| Authors: | Murakami Chikako Yamazaki Takayuki Hanashima Shinya Takahashi Shunya Takemura Masaharu Yoshida Shonen Ohta Keisuke Yoshida Hiromi Sugawara Fumio Sakaguchi Kengo Mizushina Yoshiyuki |
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| Institution: | Department of Nutritional Science, Laboratory of Food & Nutritional Sciences, Kobe-Gakuin University, Nishi-ku, Hyogo 651-2180, Kobe, Japan. |
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| Abstract: | Sulfo-glycolipids in the class of sulfoquinovosyl diacylglycerol (SQDG) including the stereoisomers are potent inhibitors of DNA polymerase alpha and beta. However, since the alpha-configuration of SQDG with two stearic acids (alpha-SQDG-C(18)) can hardly penetrate cells, it has no cytotoxic effect. We tried and succeeded in making a permeable form, sulfoquinovosyl monoacylglycerol with a stearic acid (alpha-SQMG-C(18)) from alpha-SQDG-C(18) by hydrolysis with a pancreatic lipase. alpha-SQMG-C(18) inhibited DNA polymerase activity and was found to be a potent inhibitor of the growth of NUGC-3 cancer cells. alpha-SQMG-C(18) arrested the cell cycle at the G1 phase, and subsequently induced severe apoptosis. The arrest was correlated with an increased expression of p53 and cyclin E, indicating that alpha-SQMG-C(18) induced cell death through a p53-dependent apoptotic pathway. |
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