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Decolourisation of Remazol Brilliant Blue R via a novel Bjerkandera sp. strain
Authors:Moreira P R  Almeida-Vara E  Sena-Martins G  Polónia I  Malcata F X  Cardoso Duarte J
Institution:

a Unidade de Bioengenharia e Bioprocessos, Instituto Nacional de Engenharia e Tecnologia Industrial (INETI), Estrada do Paço do Lumiar, 22, P-1649-038, Lisboa, Portugal

b Escola Superior de Biotecnologia, Universidade Católica Portuguesa, R. Dr. António Bernardino de Almeida, P-4200-072, Porto, Portugal

c Escola Superior Agrária de Santarém, Quinta do Galinheiro, S. Pedro, P-2000-014, Santarém, Portugal

Abstract:A novel strain of Bjerkandera sp. (B33/3), with particularly high decolourisation activities upon Poly R-478 and Remazol Brilliant Blue R (RBBR) dyes, was isolated. The role of the ligninolytic extracellular enzymes produced by this strain on decolourisation of RBBR was studied in some depth. The basis of decolourisation is an enzyme-mediated process, in which the main enzyme responsible is a recently described peroxidase with capacity for oxidation of manganese, as well as veratryl alcohol and 2,6-dimethoxyphenol in a manganese-independent reaction.
Keywords:Bjerkandera sp    Remazol Brilliant Blue R  Dyes
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