A simple in vitro culture system for tracheal cartilage development |
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Authors: | Jinhyung Park Jennifer J R Zhang Ruth Choi Irene Trinh Peter C W Kim |
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Institution: | (1) Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, Toronto, ON, M5G 1X8, Canada |
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Abstract: | Semi-circular tracheal cartilage is a critical determinant of maintaining architectural integrity of the respiratory airway.
The current effort to understand the morphogenesis of tracheal cartilage is challenged by the lack of appropriate model systems.
Here we report an in vitro tracheal cartilage system using embryonic tracheal–lung explants to recapitulate in vivo tracheal
cartilage developmental processes. With modifications of a current lung culture protocol, we report a consistent in vitro
technique of culturing tracheal cartilage from primitive mouse embryonic foregut for the first time. This tracheal culture
system not only induces the formation of tracheal cartilage from the mouse embryonic foregut but also allows for the proper
patterning of the developed tracheal cartilage. Furthermore, we show that this culture technique can be applied to culturing
other types of cartilage in vertebrae, limbs, and ribs. We believe that this novel application of our in vitro culture system
will facilitate the manipulation of cartilage development under various conditions and thus enabling us to advance our current
limited knowledge on cartilage biology and development. |
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