The simultaneous production of sorbitol from fructose and gluconic acid from glucose using an oxidoreductase of Zymomonas mobilis |
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Authors: | U H Chun P L Rogers |
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Institution: | (1) Department of Biotechnology, University of NSW, P.O. Box 1, 2033 Kensington, NSW, Australia |
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Abstract: | Summary The production of sorbitol and gluconic acid by toluene-treated, permeabilized cells of Zymomonas mobilis has been evaluated. From a 60% total sugar solution (300 g/l glucose and 300 g/l fructose), a sorbitol concentration of 290 g/l and a gluconic acid concentration of 283 g/l were achieved after 15 h in a batch process using free toluene-treated cells. A continuous process with immobilized cells was developed and only a small loss of enzyme activity (less than 5%) was evident after 120 h. With a strongly basic anion exchange resin and an eluent of 0.11 M Na2B4O7/0.11 M H3BO3, good separation of sorbitol and gluconic acid was achieved. |
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