OLAND生物脱氮系统中硝化菌群16S rDNA的DGGE分析

为了考察生物脱氮系统中硝化菌群（氨氧化菌和亚硝酸氧化菌）的种群多样性及硝化菌群随溶解氧降低的种群变化规律，并建立一套行之有效的用于自养生物脱氮系统中功能微生物菌群的快速分子检测技术，采用DGGE（变性梯度凝胶电泳）分子检测技术对硝化菌群的16SrDNA的特异性PCR扩增产物进行了分析，结果表明：OLAND生物脱氮系统中氨氧化菌和亚硝酸氧化菌随溶解氧的降低表现出了不同的种群变化规律，氨氧化菌种群多样性受溶解氧的影响非常大，而非亚硝酸氧化菌的种群多样性比较单一，且不受溶解氧的影响。结合FISH（全细胞荧光原位杂交）分析结果表明，在OLAND限氧稳定运行后期，亚硝化单胞菌属（Nitrosomonas）是主要的氨氧化菌，占OLAND限氧亚硝化阶段反应器中总细菌数的72.5%左右。

DGGE Analysis of 16S rDNA of Nitrifying Bacteria in OLAND Biological N Removal System

In order to investigate the microbial community diversity of nitrifying bacteria (ammonium oxidizing bacteria and nitrite oxidizing bacteria) and their dynamic rules changed with the decrease of DO in OLAND system, and then to establish a practical molecular monitoring technology suitable for identifying the functional microbial community of autotrophic N removal system, the specific products of 16S rDNA PCR amplification of nitrifying bacteria were analyzed with DGGE technique.It was found that there was a great deal of difference between ammonium oxidizing bacteria and nitrite oxidizing bacteria on the dynamic rules of microbial community changed with the drop of DO.The DO had a greater effect on ammonium oxidizing bacteria than nitrite oxidizing bacteria.Simultaneously,at the oxygen limited nitritation stage of OLAND system,nitrosomonas was proved to be the dominant ammonium oxidizing bacteria by FISH technique, which accounted for 72.5% of total bacteria in the reactor of this stage.