The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain |
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Authors: | Iwaki Daisuke Nishitani Chiaki Mitsuzawa Hiroaki Hyakushima Naoki Sano Hitomi Kuroki Yoshio |
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Institution: | Department of Biochemistry, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-ku, Sapporo 060-8556, Japan. |
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Abstract: | CD14 has been shown to enhance Toll-like receptor 2 (TLR2)-mediated signaling in response to peptidoglycan. Anti-CD14 monoclonal antibody MEM-18, whose epitope was located at the amino acid residues 57-64, blocked the binding of sCD14 to the recombinant soluble form of the extracellular TLR2 domain (sTLR2). The deletion mutant sCD14Delta57-64 lacking the amino acid residues 57-64 failed to bind to sTLR2. Cotransfection of wild type mCD14 but not mCD14Delta57-64 with TLR2 enhanced NF-kappaB activation in response to peptidoglycan. These results indicate that the CD14 region spanning amino acids 57-64 is critical for interacting with TLR2 and enhancing TLR2-mediated peptidoglycan signaling. |
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Keywords: | CD14 Toll-like receptor 2 Peptidoglycan |
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