| 毛头鬼伞多糖CCP60a对TMV外壳蛋白的影响 |
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| 引用本文： | 吴艳兵,谢荔岩,谢联辉,林奇英.毛头鬼伞多糖CCP60a对TMV外壳蛋白的影响[J].植物资源与环境学报,2008,17(3):63-66. |
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| 作者姓名： | 吴艳兵 谢荔岩 谢联辉 林奇英 |
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| 作者单位： | 1. 福建农林大学植物病毒研究所,生物农药与化学生物学教育部重点实验室,福建,福州,350002;河南科技学院资源与环境学院,河南,新乡,453003
2. 福建农林大学植物病毒研究所,生物农药与化学生物学教育部重点实验室,福建,福州,350002 |
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| 基金项目： | 福建省教育厅专项科学基金，福建省科技厅科研项目，河南科技学院高层次人才资助项目 |
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| 摘 要： | 对毛头鬼伞（Coprinus comatus Muell．ex Fr．）子实体中的多糖CCP60a进行了提取分离及检测，研究了不同温度条件下CCP60a对烟草花叶病毒（TMV）外壳蛋白体外聚合的影响，并用Western blotting法研究了CCP60a对TMV外壳蛋白表达的影响。结果表明，经沸水浸提、乙醇分级沉淀、DEAESephadex A-25离子交换柱层析、Sepharose-6B凝胶柱层析和抗TMV活性跟踪检测可得到均一的多糖CCP60a。随温度的提高，CCP60a处理组在320nm处的吸光度值增加幅度明显小于对照组，表明CCP60a对TMV外壳蛋白体外聚合有一定的抑制作用。Western blotting检测结果显示，CCP60a可以使TMV外壳蛋白的表达明显降低。
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| 关 键 词： | 毛头鬼伞 多糖CCP60a 烟草花叶病毒(TMV) 外壳蛋白 |
Effect of polysaccharide CCP60a from Coprinus comatus on TMV coat protein |
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| WU Yan-bing,XIE Li-yan,XIE Lian-hui,LIN Qi-ying.Effect of polysaccharide CCP60a from Coprinus comatus on TMV coat protein[J].Journal of Plant Resources and Environment,2008,17(3):63-66. |
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| Authors: | WU Yan-bing XIE Li-yan XIE Lian-hui LIN Qi-ying |
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| Institution: | WU Yan-bing, XIE Li-yan, XIE Lian-hui, LIN Qi-ying( 1. Key Laboratory of Biopestieide and Chemical Biology of Ministry of Education, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Department of Resources and Environment, He＇ nan Institute of Science and Teehnology, Xinxiang 453003, China) |
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| Abstract: | The polysaccharide CCP60a was extracted and separated from the fruiting bodies of Coprinus comatus Muell. ex Fr. The effect of CCP60a on polymerization of TMV coat protein in vitro was studied under different temperatures, and the effect of CCP60a on expression of TMV coat protein was also tested by Western blotting method. The results showed that the homogeneous polysaccharide CCP60a was obtained by the boiling water extraction, ethanol precipitation, DEAE Sephadex A-25 column chromatography, Sepharose-6B gel column chromatography and anti-TMV activity examination. The increasing extent of absorbanee value （ 320 nm） of CCP60a treatment group was smaller than that of the control with increasing of temperature, this showed that CCP60a had an inhibition effect on polymerization of TMV coat protein in vitro. The result of Western blotting test also indicated that CCP60a could obviously reduced the expression of TMV coat protein. |
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| Keywords: | Coprinus comatus Muell ex Fr polysaccharide CCP60a tobacco mosaic virus （TMV） coat protein |
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