Effects of hygromycin on cotton cultures and its application in <Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated cotton transformation |
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| Authors: | Zhao-Hong Meng Ai-Hua Liang Wei-Cai Yang |
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| Institution: | (1) Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People’s Republic of China;(2) Temasek Life Science Laboratory, National University of Singapore, 1 Research Link, Singapore, 11764, Singapore;(3) Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China |
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| Abstract: | We have evaluated the effects of the antibiotic hygromycin B on cotton (Gossypium hirsutum L.) callus induction, callus proliferation, and seed germination. Nontransgenic cotyledon and hypocotyl showed obvious variance
in tolerance to hygromycin. Cotyledons were more sensitive to hygromycin than hypocotyls. Hygromycin at 7.5 and 20 mg l−1 completely inhibited callus initiation from cotyledon and hypocotyl explants, respectively. Nontransformed calli did not
grow on media supplemented with 10 mg l−1 hygromycin and were killed at 15 mg l−1. In seed germination assay, the presence of 20 mg l−1 hygromycin significantly suppressed shoot and root elongation of seedlings. This hygromycin concentration was applied to
select regenerated transgenic plantlets and their progenies. Based on these results, we developed an efficient hygromycin
selection protocol for Agrobacterium-mediated cotton transformation and regeneration. |
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| Keywords: | Cotton transformation Cotyledon Hygromycin selection Hypocotyls |
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