使用RAPD分析辨别印度半岛的五种结鱼

用RAPD分析研究了结鱼种组(黄鳍结鱼Tor putitora,结鱼Tor tor,库德里结鱼Tor khudree,Tor mosalmahanadicus和墨脱四■魮Neolissochilus hexagonolepis)5个物种的遗传关系。在所测试的69个随机引物中,11个引物能够在所有5个物种中扩增出稳定的条带。RAPD带型显示,综合使用这些RAPD标记能够区分这5个物种,但Tor mosal mahanadicus和黄鳍结鱼享有相似的带型。UPGMA分析揭示出3个独特的分支,第一支由黄鳍结鱼、Tor mosal mahanadicus和结鱼组成,第二支是库德里结鱼,第三支是墨脱四■魮。Tor mosal mahanadicus的分类地位在不同学者间存在分歧,被认为是库德里结鱼或结鱼的亚种,但我们的结果表明,相对而言,Mahanadi河中的Tor mosal mahanadicus与黄鳍结鱼的进化关系更近,因此有必要对其系统分类地位进行重新评估。

Molecular discrimination of five Mahseer species from Indian peninsula using RAPD analysis

The genetic relatedness between five species of Mahseer group(Tor putitora,Tor tor,Tor khudree,Tor mosal mahanadicus and Neolissochilus hexagonolepis)was examined by Random Amplified Polymorphic DNA(RAPD)analysis for the first time.Out of the sixty-nine random primers tested,eleven primers generated scoreable patterns in all the five species.The comparative RAPD profiles revealed that the combination of RAPD markers could discriminate the species,except between T.mosal mahanadicus and T.putitora,which had a similar RAPD profile.UPGMA analysis depicted three distinct clusters;one formed by T.putitora,T.mosal mahanadicus and T.tor,the second by T.khudree and the third of Neolissochilus hexagonolepis.The taxonomic status of T.mosal mahanadicus is the subject of disagreement between authors;it has been considered as a subspecies of T.khudree and T.tor.The results demonstrated that the T.mosal mahanadicus of the river Mahanadi is closer to T.putitora than to the other Tor species studied and reassessment of its systematic position is required.