| 理化诱变引起的小麦再生株染色体变异 |
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| 引用本文： | 胡天赐,高明尉,梁竹青. 理化诱变引起的小麦再生株染色体变异[J]. 分子细胞生物学报, 1993, 0(1) |
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| 作者姓名： | 胡天赐 高明尉 梁竹青 |
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| 作者单位： | 浙江农业大学原子核农业科学研究所(胡天赐，高明尉)，浙江农业大学原子核农业科学研究所(梁竹青) |
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| 摘 要： | 小麦幼胚和愈伤组织经理化诱变后形成的再生株M_1R_1和C_1R_1,具有比R_1株高的染色体变异频率,其染色体数目变化于35—46之间,但无倍数变异。理化诱变处理提高了再生株中含非整倍体、棒状二价体、单价体、等臂环和四价体的细胞频率,其中以1000伦琴γ射线照射未成熟胚处理的再生株染色体变异频率为最高。其次为2 mmol/L叠氮化钠处理愈伤组织的再生株。经过二倍体和单倍体选择,染色体变异大为减少,但仍保有较高频率的单价体。
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| 关 键 词： | 普通小麦 组织培养 体细胞无性系变异 染色体变异 理化诱变 |
CHROMOSOMAL VARIATIONS IN REGENERATED PLANTS FROM MUTAGENIZED IMMATURE-EMBRYOS AND CALLI IN WHEAT |
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| Hu Tian-ci Gao Ming-wei Liang Zhu-qing. CHROMOSOMAL VARIATIONS IN REGENERATED PLANTS FROM MUTAGENIZED IMMATURE-EMBRYOS AND CALLI IN WHEAT[J]. Journal of Molecular Cell Biology, 1993, 0(1) |
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| Authors: | Hu Tian-ci Gao Ming-wei Liang Zhu-qing |
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| Abstract: | The regenerated plants derived fromimmature-embryo culture and calli treatedwith physical and chemical mutagens sho-wed greater chromosomal variation,thechromosome number ranged between 35 and46.In all of the regenerated plants,noploidy variation was found.Mutationtreatment increased the frequency of cellswith chromosome aberration inculudinganeuploid,clubbed bivalent,univalent,iso-ring and polyvalent.Immature-embryoirrdiated with 1000 R gamma-rays beforeinoculation had the highest frequency ofchromosomal changes,followed by thecallus treated with 2 mmol/L NaN_3.InM_2R_2 aod C_2R_2 generation,the chromosmalvariation decreased due to diploid andhaploid selection,but the frequency of cellswith univalent was still higher. |
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| Keywords: | Wheat (Triticum aestivum).Induced mutation.Tissue ulture.Somaclonal variation.Chromosomal variation. |
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