Linkage map of Japanese black pine based on AFLP and RAPD markers including markers linked to resistance against the pine needle gall midge |
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| Authors: | E Hayashi T Kondo K Terada N Kuramoto Y Goto M Okamura H Kawasaki |
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| Institution: | (1) Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: ehayas@nftbc.affrc.go.jp Fax: +81-293-32-7306, JP;(2) Tohoku Breeding Office, Forest Tree Breeding Center, Takisawa-mura, Iwate 020-0173, Japan, JP |
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| Abstract: | Macrogametophytes derived from the seeds of a tree resistant to pine needle gall midge (PGM) were analyzed using amplified
fragment length polymorphism (AFLP). A total of 244 segregating loci were detected among 71 macrogametophytes. Combining the
AFLP results with previously reported segregation data for 127 random amplified polymorphic DNA (RAPD) markers, 157 AFLP and
50 RAPD markers with confirmed map positions were assigned to 20 linkage groups and three pairs covering 2085.5 cM with an
average distance of 10.1 cM. The total map distance covers about 77.1–78.4% of the total genome, estimated to be approximately
2665–2719 cM in length. Thus, using AFLP markers, the previous RAPD map of this tree was improved in terms of the average
distance between markers, the total map distance, and coverage of the genome. Three RAPD markers linked to a gene associated
with resistance to PGM were also located on this map.
Rceived: 14 April 2000 / Accepted: 21 August 2000 |
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| Keywords: | Pinus thunbergii Macrogametophyte Linkage map AFLP RAPD |
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