Further characterization of [3H]fulunitrazepam binding sites on cultured mouse astroglia |
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Authors: | M Tardy C Fages G Dupre M F Costa J Bardakdjian B Rolland |
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Institution: | (1) Department de Biochimie, C.H.U. Henri Mondor, 51, av. de Lattre de Tassigny, 94010 Creteil Cedex, France |
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Abstract: | Astroglial cells in primary cultures bind 3H]flunitrazepam with a high affinity on a single type of site and on a number of binding sites which increased during astroglial growth and differentiation. These binding sites show a particular pharmacological spectrum characterized by an inhibition of high affinity by RO-5-4864 (4 -chlorodiazepam), an anticonvulsant of the benzodiazepine family and by an inhibition of binding of lower affinities by diazepam clonazepam and clobazam. RO-5-4864 and clonazepam compete for the same binding site in astroglia. The heat stability and the hormonal modulation by thyroxine are similar for astroglia and neuronal-cells. Benxodiazepines modulate the astroglial 5-HT receptor. Such an effect could be a possible physiological response to benzodiazepines for astroglial cells in primary cultures. |
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