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The isolation of viable egg cells of wheat (Triticum aestivum L.) |
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| Authors: | Miklós Kovács Beáta Barnabás Erhard Kranz |
| Affiliation: | (1) Cell Biology Department, Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462 Martonvásár, Hungary;(2) University of Hamburg, Centre for Applied Plant Molecular Biology, AMP II, Institute of General Botany, Ohnhorststr. 18, D-22609 Hamburg, Germany |
| Abstract: | The isolation of viable egg cells of wheat has been achieved without enzymatic maceration of the ovules. 2,4-D applied to the stigmas resulted 3 to 7 days later in soft ovule tissues which disintegrated upon mechanical manipulation. The isolated egg cells were viable even 2 h after isolation. Their morphology corresponded to that of the in situ egg cells. The mean isolation frequency was 20% (two egg cells per ten ovules). |
| Keywords: | Egg cell apparatus in situ Egg cell isolation Wheat |
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