New CMS types in Plantago lanceolata and their relatedness |
| |
Authors: | A A de Haan A C Mateman P J Van Dijk J M M Van Damme |
| |
Institution: | (1) NIOO-CTO, PO box 40, 6666 ZG Heteren, The Netherlands, NL |
| |
Abstract: | Mitochondrial variation in Plantago lanceolata was used to detect new CMS types. Directional reciprocal crosses were made between plants which differed in mtDNA restriction
patterns. Differential segregation of male steriles in reciprocal crosses indicated that the parents differed in CMS type.
MtDNA variation revealed nine RFLP patterns, which could be categorised according to the sex phenotype of the plants as MS1
(brown-anther type), MS2 (petaloid flower type) and MS3 (more yellow anthers than MS1). A single mtDNA pattern was found within
MS1, six mtDNA patterns were found within the MS2 group, and two mtDNA patterns were found within hermaphrodites which segregated
MS3 in the crosses. MS1 and MS2 are known to represent different CMS types, CMSI and CMSII. In reciprocal crosses between
plants with different mtDNA patterns within the MS2 group, different ratios of male steriles segregated in the crosses, indicating
that the parents differed in CMS type. Within the MS2 group two CMS types were found, designated CMSIIa and b. Finally, the
sex phenotype H/MS3 turned out to be a different type. From previous studies it was known that the MS3 phenotype can also
occur in CMSI and CMSII types, hence it is unclear whether MS3 is diagnostic for CMSIII. Since the data in this study cannot
distinguish between the new type being a fully restored new CMS type or a ‘Normal’ cytoplasm, it was denoted as CMSIII. In
total, four CMS types were found in the material studied. CtDNA variation was screened and three chloroplast haplotypes were
identified. Two haplotypes were associated with CMSI plants, and one haplotype with the other CMS types. The ctDNA variation
indicated that the CMSI type is widespread within the species, due to migration rather than to recurrent mutation. This may
lead to the conclusion that only a limited number of CMS types are successful.
Received: 9 August 1996 / Accepted: 6 September 1996 |
| |
Keywords: | Plantago lanceolata Gynodioecy CMS ctDNA Reciprocal crosses |
本文献已被 SpringerLink 等数据库收录! |
|