Serum and insulin inhibit cell death induced by cycloheximide in the human breast cancer cell line MCF-7 |
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Authors: | Avraham Geier Rachel Beery Michal Haimshon Rina Hemi Bruno Lunenfeld |
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Institution: | (1) Institute of Endocrinology, Chaim Sheba Medical Center, 52621 Tel Hashomer, Israel |
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Abstract: | Continuous exposure of cells to cycloheximide (CHM) terminates in cell death. This may result from CHM’s inhibition of protein
synthesis. In the present study we investigated the effect of serum and insulin on cell death induced by CHM in the human
breast cancer cell line MCF-7, and correlated this effect to the inhibition of protein synthesis. Cell death was evaluated
by measuring either dead cells by the trypan blue dye exclusion test or by the release of lactic dehydrogenase into the culture
medium. CHM (0.1 to 50 μg/ml) was shown to induce cell death in a time- and concentration-dependent manner. Including either
fetal bovine serum or insulin in the culture medium inhibited this cell death in a concentration-dependent manner. Protein
synthesis as measured by 3H]leucine incorporation was inhibited by the increasing concentration of CHM, However, fetal bovine serum and insulin did
not alter the protein synthesis inhibition rate induced by CHM. These results indicate that inhibition of protein synthesis
is not enough for cell death to proceed. Insulin or factors present in serum may stabilize some crucial cell proteins (key
enzymes, cytoskeletal or membrane components) which are vital for cell life. |
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Keywords: | serum insulin cell death cycloheximide |
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