Discovery of a new HBB haplotype <Emphasis Type="Italic">w2</Emphasis> in a wild-derived house mouse, <Emphasis Type="Italic">Mus musculus</Emphasis> |
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| Authors: | Jun J Sato Akio Shinohara Nobumoto Miyashita Chihiro Koshimoto Kimiyuki Tsuchiya Ikuyo Nakahara Tetsuo Morita Hiromichi Yonekawa Kazuo Moriwaki Yasunori Yamaguchi |
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| Institution: | (1) Laboratory of Animal Cell Technology, Faculty of Life Science and Technology, Fukuyama University, Higashimura-cho, Aza, Sanzo, Fukuyama 729-0292, Japan;(2) Bio-resource Division, Department of Biotechnology, Frontier Science Research Center, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan;(3) Division of Animal Experiment, Life Science Research Center, Institute of Research Promotion, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan;(4) Laboratory of Bioresources, Applied Biology Co., Ltd, Minato-ku, Tokyo 107-0062, Japan;(5) Division of Animal Science, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan;(6) Tokyo Metropolitan Institute of Medical Science (RINSHOKEN), 18-22, Honkomagome 3-chome, Bunkyo-ku, Tokyo 113-8613, Japan;(7) RIKEN Tsukuba Institute, RIKEN Bioresource Center, Kouyadai 3-1-1, Tsukuba 305-0074, Japan;(8) Present address: Miyazaki Kita Police Station, Miyazaki Prefectural Police, 4-8 Nishiki Honmachi, Miyazaki 880-0818, Japan |
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| Abstract: | Genetic characterization of a wild-derived house mouse, Mus musculus, originally collected near Lake Balkhash in the Republic of Kazakhstan, was performed by examining protein polymorphisms
and nucleotide sequences for the hemoglobin beta chain (HBB) subunits. Protein electrophoresis, which was performed on a cellulose-acetate
plate, showed an independent mobility pattern representing a new, previously undiscovered haplotype. Neighbor-joining analyses
of the HBB adult genes, i.e., HBB-T1 and HBB-T2, and the intergenic spacer region showed that the Lake Balkhash mouse possessed
genomic components that were mixed from different haplotypes. Compared to the previously determined HBB haplotypes, d, p, and w1, the HBB-T1 gene and ca. 11 kb of the spacer region were most similar to the w1 haplotype; however, the remainder of the spacer region and the HBB-T2 gene were most similar to the d haplotype but may represent a still uncharacterized and divergent haplotype. The recombination event is predicted to have
occurred 2.5 kb upstream of the HBB-T2 gene and may have occurred through intersubspecific hybridization between mice of the
musculus subspecies group (with the w1 haplotype) and the castaneus subspecies group (with the d-like haplotype). Alternatively, an unknown subspecies group that is equidistantly divergent from each of these subspecies
groups may have been involved. Our findings suggest reticulate evolution among the subspecies groups during the evolution
of M. musculus. |
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