Statistical modeling of protein spray drying at the lab scale |
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Authors: | Prinn Kristin B Costantino Henry R Tracy Mark |
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Institution: | (1) Alkermes, Inc, 64 Sidney Street, 02139 Cambridge, MA |
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Abstract: | The objective of this study was to examine the effects of formulation and process variables on particle size and other characteristics
of a spray-dried model protein, bovine serum albumin (BSA), using a partial factorial design for experiments. Formulation
variables tested include concentration and zinc:protein complexation ratio. Process variables explored were inlet temperature,
liquid feed rate, drying air flow rate, and atomizing nitrogen pressure on a lab-scale spray dryer. Statistical data analysis
was used to determine F ratios for each of the inputs, which provided a means of ranking the importance of variables relative
to one another for each powder characteristic of interest. It was found that protein concentration and atomizing nitrogen
pressure had the greatest effects on the particle size of the protein powder. For determining product yield, results showed
that protein concentration was the critical variable. Finally, the outlet temperature was mostly influenced by inlet temperature
and liquid feed rate. Mathematical models based on these input-output relationships were constructed; these models provide
insight into some of the controllable variables of the spray-drying process.
Published: March 20, 2002 |
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Keywords: | Bovine serum albumin (BSA) Particle size Spray drying outlet temperature Yield |
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