Chemical and genetic analysis of 16S ribosomal RNA in Escherichia coli |
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| Authors: | Akira Muto Renkichi Takata Syozo Osawa |
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| Affiliation: | (1) Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-cho, Hiroshima, Japan;(2) Present address: Institut de Biologie Moléculaire, Université de Genève, 30, Quai de L'École-de-Médecine, Genève |
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| Abstract: | Summary Comparative chemical analyses of oligonucleotides arising from pancreatic RNase digestions of 16S ribosomal RNAs from Escherichia coli strains K12 and B(H) showed that a decanucleotide fragment, (5Ap,4Gp)Cp, could be detected exclusively in strain K12 but not in strain B(H), in spite of gross similarity of nucleotide distributions between the two strains.The K12-specific oligonucleotide could not be cotransduced with streptomycin and/or spectinomycin resistant markers from K12 to B(H) by phage Plkc, indicating that the genes specifying 16S ribosomal RNA are not closely linked to these markers on the chromosome. |
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