aspS encoding an unusual aspartyl protease from Sclerotinia sclerotiorum is expressed during phytopathogenesis |
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Authors: | Poussereau N Gente S Rascle C Billon-Grand G Fèvre M |
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Affiliation: | Laboratoire de Biologie Cellulaire Fongique [Bat. 405], ERS 2009 CNRS INSA LYON1, Microbiologie et Génétique, 43 bd 11 novembre 1918, 69622 Cedex, Villeurbanne, France |
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Abstract: | The gene aspS encoding an aspartyl protease has been cloned from Sclerotinia sclerotiorum by screening a genomic library with a PCR-amplified fragment of the gene. The open reading frame of 1368 bp interrupted by one intron would encode a preproprotein of 435 amino acids. The catalytic aspartyl residues characteristic of aspartyl proteases are conserved; however, the active-site motif (DSG) in the N-terminal lobe is unusual in that Ser replaced Thr used in the active-site motif (DTG) of the C-terminal lobe and in all other fungal aspartyl proteases. RT-PCR revealed that aspS expression in axenic culture is not subjected to catabolite repression and demonstrated that aspS is expressed from the beginning of infection of sunflower cotyledons. |
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Keywords: | Sclerotinia sclerotiorum Aspartyl protease gene Gene expression Plant pathogenesis |
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