Isolation of the inorganic pyrophosphatase from brewer's yeast and studies on the localization of this enzyme in brewer's and baker's yeast.

• 1.1. Inorganic pyrophosphatase is mainly located in the hyaloplasm for both brewer's and baker's yeast.
• 2.2. Extensive purification of brewer's yeast pyrophosphatase results in the separation of three active forms of the enzyme.
• 3.3. The isoelectric point of all the three enzymes is close to 5.0.
• 4.4. The molecular weight of the most active pyrophosphatase is about 60,000.