Comparison of small-scale methods for the rapid extraction of plant DNA suitable for PCR analysis |
| |
| Authors: | Hilary J. Rogers Nigel A. Burns Helen C. Parkes |
| |
| Affiliation: | (1) School of Pure and Applied Biology, University of Wales, College of Cardiff, PO Box 915, CF1 3TL Cardiff, U.K.;(2) Analytical Molecular Biology Group, Laboratory of the Government Chemist, Queens Road, TW11 OLY Teddington, Middlesex, U.K. |
| |
| Abstract: | We present results from a comparison of six methods for rapid DNA extraction from leaf and other plant tissues. We have used samples from six plant species in our study, including both crop species and their wild relatives. The success of the methods is assessed by PCR of the DNA using conserved primers, and the applicability of the different methods to particular species and tissues is assessed. The speed, reliability, convenience, and potential for further improvement of the methods are also discussed. |
| |
| Keywords: | DNA isolation leaf discs PCR RAPD |
| 本文献已被 SpringerLink 等数据库收录！ |
|
