Monoclonal anti-GD3 antibodies selectively inhibit the proliferation of human malignant glioma cells in vitro |
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Authors: | Kristina M Hedberg Birgitta Dellheden Carol J Wikstrand Pam Fredman |
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Institution: | (1) Göteborg University, Sahlgrenska University Hospital/Mölndal, Institute of Clinical Neuroscience, Section of Experimental Neuroscience, S-431 80 Mölndal, Sweden;(2) Department of Pathology, Duke University Medical Center, Durham, N.C. 27710, USA |
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Abstract: | The frequently occurring alteration of ganglioside expression in tumor cells has been implicated to play a role in the uncontrolled growth of these cells; antibodies to such gangliosides might affect tumor cell growth. We have studied the effect of IgM monoclonal antibodies to two glioma-associated gangliosides, GD3 and GM2, on cell proliferation of four human glioma cell lines and one renal tumor cell line. Of the two anti-ganglioside antibodies tested, only the anti-GD3 antibody resulted in a significant (p<0.005) inhibition of cell proliferation as measured by thymidine incorporation and Brd-U labeling, after 24emsp4 ]h incubation. The effect was not dependent on any serum factor and no increased cell death was observed. All cell lines contained higher or similar amounts of GM2 than GD3, and both antigens were shown to be expressed on the cell surface and accessible to antibodies. The selective effect of anti-GD3 antibodies as contrasted to the inactivity of anti-GM2 antibodies suggests a possible role for ganglioside GD3 in tumor cell proliferation. |
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Keywords: | carbohydrate antigens gangliosides glioma monoclonal antibodies proliferation |
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