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Isolation and Characterization of Matrix Associated Region DNA Fragments in Rice (Oryza sativa L.) |
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| Authors: | Nomura Koji; Saito Wataru; Ono Kimiyo; Moriyama Hiromitsu; Takahashi Seiko; Inoue Masayasu; Masuda Kiyoshi |
| Institution: | 1 Institute of Agriculture and Forestry, University of Tsukuba Tsukuba, Ibaraki, 305 Japan 2 Institute of Biological Science, University of Tsukuba Tsukuba, Ibaraki, 305 Japan 3 Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo, 183 Japan 4 Division of Cell Biology, Akita Prefecturat College of Agriculture Ohgata, Akita, 010-04 Japan 5 Department of Horticultural Science, Faculty of Agriculture, Hokkaido University Sapporo, 060 Japan |
| Abstract: | To investigate the interactions between chromosomal DNA andnuclear matrices in higher plants, matrix associated regions(MARs) of rice (Oryza sativa L.) DNAs were cloned. First, weprepared nuclear matrices from isolated nuclei by digestingthem with EcoRl and then extracting with 2 M NaCl. About 6%of the total DNA remained in the nuclear matrices after thisdigestion and extraction. The residual DNA fragments in thenuclear matrices were cloned. Some of the cloned DNA fragmentsshowed binding to certain nuclear proteins. One of the MAR fragmentscontained sequences related to known consensus motifs and ahairpin loop structure. A method is presented for isolationof matrix associated region (MAR) DNAs from plant cells. (Received January 13, 1997; Accepted July 10, 1997) |
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