A male and hermaphrodite specific RAPD marker for papaya ( Carica papayaL.) |
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| Authors: | Urasaki N Tokumoto M Tarora K Ban Y Kayano T Tanaka H Oku H Chinen I Terauchi R |
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| Institution: | (1) Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, Japan, JP;(2) Okinawa Prefectural Agricultural Experiment Station, 4-222 Sakiyama, Naha, Okinawa 903-0814, Japan e-mail: urasaki@nousi.pref.okinawa.jp Tel.: +81-98-884-9900, Fax: +81-98-885-8000, JP;(3) National Institute of Agrobiological Resources, Kannondai, Tsukuba 305-8602, Japan, JP;(4) Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, 1, senbaru, Nishihara, Okinawa 903-0213, Japan, JP |
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| Abstract: | The random amplified polymorphic DNA (RAPD) technique was used to determine the sex of a dioecious species, Carica papaya L., with three sex types, male, female and hermaphrodite. A 450 bp marker fragment, named PSDM(Papaya Sex Determination Marker),
exists in all male and hermaphrodite plants but not in the female plants so far analyzed. The DNA sequence of PSDM exhibited
no significant similarity to previously reported sequences. A sequence-characterized amplified region (SCAR) marker, SCARps,
was developed from PSDM to determine the sex of papaya. Southern hybridization, using PSDM as a probe, showed that PSDM exists
in the male and hermaphrodite genomes, but not in the female genome. This result strongly suggests that PSDM is located on
the chromosome region that is specific to the male and the hermaphrodite. SCARps is a suitable marker for the precise and
rapid diagnosis of sex in papaya.
Received: 1 February 2001 / Accepted: 22 May 2001 |
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| Keywords: | RAPD Sex determination Carica papaya Dioecious plant SCAR marker |
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