Electrolytic regeneration of NADH from NAD with a liquid crystal membrane electrode |
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Authors: | Masuo Aizawa Shuichi Suzuki Masahiko Kubo |
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Affiliation: | Research Laboratory of Resources of Utilization, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, Japan |
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Abstract: | Enzymatically active NADH was electrolytically regenerated from its oxidized from (NAD+) with a liquid crystal membrane electrode made by coating a hanging drop or a pool type of mercury electrode with a cholesteryl oleate membrane which could be in the liquid crystal state. A cholesteryl oleate membrane was employed to prevent dimerization of the NAD radical supposed to be an intermediate of electrolytic reduction of NAD+. |
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