Isolation and characterization of microsatellite markers for Cycas hainanensis C. J. Chen (Cycadaceae) |
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| Authors: | Min Zhang Zheng-Feng Wang Shu-Guang Jian Wan-Hui Ye Hong-Lin Cao Peng Zhu Ling Li |
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| Institution: | (1) Guangdong Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences, XingKe Route 723, TianHe, Guangzhou, 510650, People’s Republic of China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China |
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| Abstract: | Cycas hainanensis is an endangered cycad species endemic to Hainan Island, China. To enrich our scientific conservation for this species, we
developed eight microsatellite markers using repetitive DNA enriched libraries. The number of alleles per microsatellite locus
varied from 8 to 17. The expected (H
E
) and observed (H
O
) heterozygosities varied from 0.4754 to 0.8846 and from 0.3636 to 0.9600, respectively. These markers will be employed to
determine whether the ex situ C. hainanensis individuals in Sourh China Botanical Garden capture a representative portion of genetic diversity of the wild populations. |
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| Keywords: | Conservation Cycas hainanensis Endangered Genetic marker Microsatellite |
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