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Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum |
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| Authors: | Dr Michael J Rutten Robert G Garrison C Diane Moore A Max Fiskin Laurence Y Cheung |
| Institution: | (1) Department of Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA;(2) Department of Microbiology, University of Kansas Medical Center, Kansas City, Kansas, USA;(3) Department of Surgery, University of Kansas Medical Center, Kansas City, Kansas, USA;(4) V.A. Medical Center, Medical Research Service, Kansas City, Missouri, USA;(5) V.A. Medical Center, 4801 Linwood Boulevard, 64128 Kansas City, Missouri, USA |
| Abstract: | Summary Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific localization of alkaline phosphatase activity to the plasma membrane. Treatment of the tissues with levamisole (an alkaline phosphatase inhibitor) did not markedly reduce the specific alkaline phosphatase activity. Specific lead deposition was reduced by removal of the substrate from the reaction mixture. The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis. |
| Keywords: | Alkaline phosphatase Cytochemistry Endoplasmic reticulum smooth Gastrin G cell Golgi complex Necturus maculosus (Urodela) |
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