Exosome secretion of dendritic cells is regulated by Hrs, an ESCRT-0 protein |
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Authors: | Keiichi Tamai Nobuyuki Tanaka Takashi Nakano Yasuteru Kondo Masaaki Shiina Tomoaki Hoshino Yoshiyuki Ueno Kazuo Sugamura |
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Institution: | a Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan b Department of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan c Department of Cancer Science, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan d Division of Immunology, Miyagi Cancer Center Research Institute, Natori, Miyagi 981-1293, Japan e Department of Preventive and Social Medicine, Osaka Medical College, Takatsuki, Osaka 569-8686, Japan f Division of Respirology, Neurology, and Rheumatology, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan |
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Abstract: | Exosomes are nanovesicles derived from multivesicular bodies (MVBs) in antigen-presenting cells. The components of the ESCRT (endosomal sorting complex required for transport) pathway are critical for the formation of MVBs, however the relationship between the ESCRT pathway and the secretion of exosomes remains unclear. We here demonstrate that Hrs, an ESCRT-0 protein, is required for fascilitating the secretion of exosomes in dendritic cells (DCs). Ultrastructural analyses showed typical saucer-shaped exosomes in the culture supernatant from both the control and Hrs-depleted DCs. However, the amount of exosome secretion was significantly decreased in Hrs-depleted DCs following stimulations with ovalbumin (OVA) as well as calcium ionophore. Antigen-presentation activity was also suppressed in exsosomes purified from Hrs-depleted DCs, while no alteration in OVA degradation was seen in Hrs-depleted DCs. These data indicated that Hrs is involved in the regulation of antigen-presentation activity through the exosome secretion. |
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Keywords: | Dendritic cells ESCRT Exosomes Hrs |
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